Figure 3.

Flow cytometry analysis of cell proliferation and death in transduced N-TERT cells. (a) Cells transduced with eGFP or SPINK5/eGFP expressing viral vector were treated with EdU. Examples of the distribution of cell populations in different stages of the cell cycles are shown. There were no significant differences between groups, suggesting that lentiviral transduction and LEKTI expression do not affect cell turnover. (b) Cell viability of transduced N-TERT cells was analyzed using propidium iodide staining and flow cytometry. The distribution of live and dead cells in the eGFP⁺, transduced cell populations is shown. There were no significant differences in cell death or survival between groups indicating that transduction with vectors encoding SPINK5/eGFP does not influence cell survival. EdU, 5-ethynyl-2′-deoxyuridine; eGFP, enhanced green fluorescent protein; LEKTI, lymphoepithelial Kazal-type-related inhibitor.