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. 2010 Nov 23;19(2):395–399. doi: 10.1038/mt.2010.254

Figure 2.

Figure 2

Biochemical detection of miR-143 in the MV-rich fraction from THP-1 macrophages after transfection with miR-143BP and incubation in serum-free medium. Detection was made by performing the TaqMan miRNA assay using real-time PCR and western blot analysis of TSG101. (a) The relative amount of miR-143 is shown. The miR-143 level in the NC control (C) is indicated as “1”. miR-21 was used as an internal control. (b) The relative amounts of GAPDH and β-actin are shown. (c) Western blot analysis of TSG101. Ten micrograms of protein from THP-1 cells (macrophages) or the MV-rich fraction was loaded into each lane. The ratios of Tsg101/β-actin, which were evaluated by densitometry, are also shown. The procedure for preparation of the MV-rich fraction is described in Materials and Methods section. MV, microvesicle; THP, human acute monocytic leukemia cell line.