Figure 6.

Oncolytic virus-mediated protein presentation to CD8⁺ T-cells is sufficient for tumor cell death. Female wt C57BL/6 mice were subcutaneously vaccinated with three injections of either 5 × 10⁸ pfu VSVΔM51 or 2 × 10⁷ pfu KM100. Ten days later, the local superficial inguinal lymph nodes and spleens were harvested, and MAC-sorted CD8α⁺ pooled splenocytes were cocultured in the presence of NOP32 cells infected with the corresponding oncolytic virus. (a) IFN-γ staining of CD8⁺ T-cells cocultured with VSVΔM51-infected (left panel) or KM100-infected NOP32 cells (right panel) at the indicated multiplicities of infection (MOIs). (b) Cellular viability of VSVΔM51- or KM100-infected NOP32 cells cocultured with CD8⁺ T-cells from a as determined by 5-carboxyfluorescein diacetate-acetoxymethyl ester staining for membrane integrity (left panel) and alamar Blue staining for cellular metabolism (right panel). Errors bars denote SE of experimental means. Data are representative of two experiments. PBS, phosphate-buffered saline; VSV, vesicular stomatitis virus, IFN, interferon.