Skip to main content
. 2010 Dec 7;19(2):234–242. doi: 10.1038/mt.2010.273

Figure 7.

Figure 7

Structural integrity of second order neurons and synaptic contacts in treated retinas. Left column, green: PSD95 that stains PR synaptic terminals in the outer plexiform layer (OPL); red: Calbindin staining of horizontal cells; in treated rd10 retinas, connections between horizontal cells and photoreceptor are still visible. Red puncta, corresponding to fine dendritic tips of horizontal cells (HC), are observed while penetrating the synaptic cavity of photoreceptor terminals. Middle column, green: Calbindin staining of horizontal cells highlights the preservation of complexity of the dendrites and axonal arborizations of these neurons (arrows) in treated rd10 retina (middle), compared to the regressed arborizations (arrow) of the untreated rd10 retina (bottom). Right column, green: rod bipolar cells (RB) specific PKC staining; red: PR synaptic terminals specific PSD95 Ab staining. PSD95 labeling of photoreceptor synaptic terminals in the OPL is still clearly visible in treated rd10 retina (middle). Rod bipolar cells also show a rich complement of dendrites intermingled with PR terminal (middle) compared to regressed dendrites and scant PR endings observed in the untreated rd10 counterparts (bottom). T, treated; U; untreated, WT; wild type.