Skip to main content
. 2011 Feb 1;25(3):238–250. doi: 10.1101/gad.607311

Figure 3.

Figure 3.

EOMES expression is regulated by pluripotency factors during DE specification. (A) ChIP-qPCR analyses show the presence of NANOG, OCT4, and SOX2 on the EOMES enhancer region in hESCs over 3 d of differentiation. (B,C) Expression of EOMES in hESCs knocked down for OCT4, SOX2, or NANOG (B), and in hESCs overexpressing GFP, OCT4, SOX2, or NANOG (C). (D) Luciferase assay showing the effect of NANOG, OCT4, or SOX2 overexpression on the transcriptional activity of the EOMES enhancer region in hESCs. (*) P < 0.01 when compared with hrGFP. (E) Expression of PS (EOMES, MIXL1, BRACHYURY, and GOOSECOID) and DE (SOX17 and FOXA2) markers in shScrambled-hESCs or shNANOG-hESCs grown in CDM-ABFLY for 1 and 3 d, respectively. All error bars indicate standard deviation of three biological replicates. See also Supplemental Figure 2.