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. 2011 Feb 1;25(3):251–262. doi: 10.1101/gad.2009211

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Figure 3. — Multipotential Erg^M/M CFU-Cs fail to sustain expansion. Changes in the absolute number of hematopoietic (CD45⁺) cells (A) and functionally defined CFU-C numbers (B) in the E8.5–E10.5 YS (Erg^+/+, n = 6; Erg^+/M, n = 13; Erg^M/M, n = 6). Quantification of changes in CD45⁺ cell numbers (C) and CFU-Cs (D) in fresh P-Sp/AGM region between E9.5–E10.5 and following 96-h explant culture of E10.5 AGM (Erg^+/+, n = 6; Erg^+/M, n = 13; Erg^M/M, n = 10). (E) CFU-C classes present in fresh E10.5 AGM (panel i) and following 96-h explant culture (panel ii); data are cumulative of five independent experiments. Representative images of transverse sections from E10.5 liver of Erg^+/+ (F) and Erg^M/M (G) embryos; blast cells are indicated by arrows, and erythroid clusters are indicated by an asterisk. Original magnification, ×400. (*) P < 0.05; (**) P < 0.01; (***) P < 0.001.