Figure 5.

Wound inflammation. MIP-2 (a) and IL-1β (b) ELISA analyses from lysates of nonwounded back skin and lysates of wound tissue isolated from CD, HFD or ob/ob mice. Protein is expressed as ng (for MIP-2) or pg (for IL-1β) per 50 μg skin or wound lysate. **P < .01; *P < .05 as indicated by the brackets. Each single experimental time point depicts the mean ± SD obtained from 8 wounds (n = 8) isolated from 4 individual animals (n = 4). (c) Immunoblots showing the presence of Cox-2 protein in nonwounded (ctrl) and wounded (1 to 11 d wd) skin in CD, HFD and ob/ob mice as indicated. Each time point depicts 8 wounds (n = 8) from 4 individual mice (n = 4). A Ponceau S (PonS) staining of the immunoblots is shown to control equal loading.