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. 2011 Jan 26;2011:132958. doi: 10.4061/2011/132958

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Copyright © 2011 Songqing Na et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Compound A induces VDR-mediated gene expression. (a) Compound A is as potent as 1,25-(OH)₂D₃ in inducing VDR-LBD-dependent gene expression. Luciferase activity (±SE) of HeLa cells transfected with Gal4-VDR-LBD in a mammalian one-hybrid setting in the presence of vehicle or various concentrations of 1,25-(OH)₂D₃ or compound A is shown. Results are in arbitrary light units obtained from experiments performed in triplicate. (b) Compound A induces VDRE-dependent expression of the rat osteocalcin promoter in osteoblasts. ROS17/2.8 cells stably transfected with rat osteocalcin reporter (OCN-Luc) were treated with various concentrations of 1,25-(OH)₂D₃ or compound A. Results are shown in percentage of the luciferase activity obtained by treating the cells with 1 μM 1,25-(OH)₂D₃. All the transfections were performed in triplicate.