Figure 6.

Nonsecosteroidal VDRM is as efficacious as 1,25-(OH)₂D₃ in modulating cytokine gene expression in activated human PBMCs. Primary cells isolated from donors were stimulated with TPA (100 ng/mL) and PHA (25 μL/mL) and treated with vehicle or 100 nM each of 1,25-(OH)₂D₃ and compound A for 24 hours. Taqman Q-PCR was performed on RNA obtained form vehicle-treated or VDR ligand-treated samples using primer pairs and probes for IL-2, IL-4, IL-10, IFN-γ, TNF-α, GATA3, and GAPDH. The amount of IL-2, IL-4, IL-10, IFN-γ, TNF-α, and GATA3 transcripts relative to GAPDH transcripts is shown as mean ± SE of quadruplicate experiments.