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. 2011 Jan 13;4:3. doi: 10.1186/1755-1536-4-3

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Copyright ©2011 Bitencourt et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Hyaluronidase (HYAL) modulates cytokines in the lung parenchyma. Supernatants were prepared from the lung parenchyma obtained from C57Bl/6 mice treated after 96 h of inoculation with 16 U of bovine testicular HYAL or phosphate buffered saline (PBS). Interleukin (IL)-1, IL-2, IL-4, IL-5, IL-10, IL-12, tumour necrosis factor (TNF)-α and interferon (IFN)-γ were assayed by ELISA. (A) IL-1, P = 0.001; (B) IL-2, P = 0.01; (C) IL-4, P = 0.004; (D) IL-5, P = 0.006; (E) IL-10, P = 0.08; (F) IL-12, P = 0.54; (G) TNF-α, P = 0.02; (H) IFN-γ, P = 0.07; (A-H) = values represent means ± SEM compared to PBS; n = 5, t test was used.