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. Author manuscript; available in PMC: 2011 Feb 8.
Published in final edited form as: J Immunol. 2009 Dec 11;184(2):965–974. doi: 10.4049/jimmunol.0902840

FIGURE 4.

FIGURE 4

Overexpressing IκBα mimics vitamin D effects. A. hTBE cells were infected with GFP labelled adenovirus vector (MOI 10) for 24 h and transgene expression assessed with fluorescent microscopy. About 80% of the cells were found to express the GFP labelled adenovirus vector. B. and C. hTBE cells were infected with either empty adenovirus vector or an adenovirus vector overexpressing IκBα (MOI 10) for 24 h and then infected with RSV (MOI 1-2) or stimulated with IFNβ (1000U) for 24 h. IFNβ, CXCL10, MxA and ISG15 mRNA was quantified using qRT-PCR. Like 1,25D, the AdIκBα vector significantly reduced RSV induction of IFNβ and CXCL10. There was a trend towards a decrease in the RSV induced antiviral effectors, MxA and ISG15, which did not reach statistical significance. Like vitamin D, the AdIκBα vector had no effects on IFNβ induced MxA and ISG15. ANOVA with Bonferroni’s test for multiple comparisons. * p<0.05 and ** p<0.01 when c/w control.