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. Author manuscript; available in PMC: 2011 Feb 8.
Published in final edited form as: J Immunol. 2009 Dec 11;184(2):965–974. doi: 10.4049/jimmunol.0902840

FIGURE 5.

FIGURE 5

Silencing the vitamin D receptor abolishes the effects of vitamin D on RSV induced IFNβ, chemokines, and interferon stimulated genes. A. hTBE cells were transfected with control siRNA or VDR siRNA for 16 or 24 h and VDR protein looked at with Western blot. The VDR siRNA successfully knocked down the VDR. B. hTBE cells were transfected with control siRNA or VDR siRNA for 24 h, pretreated with 1,25D for 16–18 h, and then infected with RSV (MOI 1-2) for 24 h. IFNβ, CXCL10, MxA and ISG15 mRNA were evaluated with qRT-PCR, CXCL10 protein with ELISA, and ISG15 protein with Western blot. Vitamin D reduced the expression of IFNβ, CXCL10, MxA and ISG15 in cells transfected with control siRNA, but had no effects in cells transfected with VDR siRNA. ANOVA with Bonferroni’s test for multiple comparisons. * p<0.05 and ** p<0.01 when c/w control.