Fig. 4.

Polarized microtubule organization in Drosophila. (A) In the oocyte, enrichment of microtubule plus ends at the posterior (right) is revealed by the plus-end-directed motor kinesin (a kinesin fusion to β-galactosidase, green). F-actin, red; nuclei, blue. Reproduced with permission (Becalska and Gavis, 2010). (B) In the pupal wing, live tracking of the microtubule plus end-binding protein Eb1 (GFP-tagged Eb1 in white) shows microtubules at a range of orientations (the colored lines), with an enrichment of plus ends oriented distally (right). Reproduced with permission (Harumoto et al., 2010). (C) In larval neuroblasts at telophase, the mitotic spindle (tubulin, green) aligns with the apical-basal axis and divides asymmetrically to produce a smaller basal cell that inherits Miranda (red). DNA, blue. Apical is up. Reproduced with permission (Giansanti et al., 2001). Scale bars: 5 μm.