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. 2011 Mar 1;138(5):861–871. doi: 10.1242/dev.055236

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Fig. 1. — Endoderm induction in human embryonic stem cell (hESC)-derived embryoid bodies (EBs). (A) Flow-cytometric analysis of CXCR4, CD117 and SOX17 on hESCs [differentiation day (d)0] and d5 EB-derived cells differentiated in the presence of activin (d5+ACT) in serum free media demonstrating efficient induction of endoderm cells using the embryoid body, serum-free differentiation system. Percentage of cells within each quadrant is indicated. (B) Quantitative PCR (QPCR) analysis of OCT4, SOX3, FOXA2 and SOX17 in d0 and d5 populations. Bars represent mean±s.d. Asterisks indicate statistical significance as determined by t-test. P=0.033 (OCT4), P=0.025 (SOX3), P=0.081 (FOXA2) and P=0.030 (SOX17). n=3.