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. 2011 Mar 1;138(5):861–871. doi: 10.1242/dev.055236

Fig. 5.

Fig. 5.

Kinetics of pancreatic lineage development. (A) Quantitative PCR (QPCR) analysis for FOXA2, SOX17, HNF1B, NGN3, NEUROD1, GCG, INS and SST in the adult pancreas (P) and at stages 1 to 5 (S1-S5) following differentiation with three different protocols: the D'Amour protocol (D'Amour et al., 2006) supplemented with noggin at stage 3 (protocol 1) and our protocol (protocol 2) with or without extended activin (Act). The protocols are depicted in Fig. S5 in the supplementary material. Expression levels are normalized to TBP. Bars represent mean±s.d. *P<0.05, **P<0.01 determined by ANOVA with Tukey's HSD test. (B) Intracellular flow-cytometric analysis of C-peptide and GCG at days 14, 20 and 22 of differentiation for protocol 1, 2 and 2+ACT, respectively. **P<0.01 determined by ANOVA with Tukey's HSD test. (C) Representative flow cytometric analysis of C-peptide and GCG at day 14 and 20 of differentiation using protocol 1 and protocol 2 showing the higher efficiency of protocol 2 in inducing endocrine cells as compared with protocol 1. Percentage of cells within each quadrant is indicated.