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. 2011 Mar 1;138(5):861–871. doi: 10.1242/dev.055236

Fig. 6.

Fig. 6.

Pancreatic differentiation of different human pluripotent stem cells (hPSCs). (A) Quantitative PCR (QPCR) analysis for INS (black histogram) and ALB (red line) at d25 of differentiation in H1-derived populations generated in the presence of FGF10 alone (d7-10; ctrl) or FGF10 and 0.25 μM (dorso 0.25), 0.50 μM (dorso 0.50) or 0.75 μM (dorso 0.75) dorsomorphin. Line and bars represent mean±s.d. n=3. (B) Immunostaining for C-peptide (red) and PDX1 (green) at d27 in H1-derived cultures. (C,D) Flow-cytometric analysis of C-peptide at d25 of differentiation in untreated (Ctrl) and dorsomorphin-treated cultures (Dorso) of H1-derived cells. Percentage represents the mean percentage cells expressing C-peptide (s.d.=0.8 for D, n=3). (E) Flow cytometric analysis measuring GFP at d22 of differentiation in INS:GFP-HES3-derived populations. Cells were differentiated in the presence of 0.75 μM dorsomorphin (Dorso). Percentage represents the mean percentage of cells expressing GFP (s.d.=2.3%, n=4). (F) Presence of GFP+ clusters in the d22 population generated from the INS:GFP-HES3 cell line differentiated in the presence of 0.75 μM dorsomorphin (Dorso). (G-I) Immunostaining for C-peptide in day 22 INS:GFP HES3-derived populations. (J) QPCR analysis for INS in d25 populations generated from the 38-2 iPS cell line. Cells were differentiated in the absence (Ctrl) or presence of 0.75 μM dorsomorphin (Dorso). Bars represent mean±s.d. Asterisk indicates statistical significance determined by t-test. P=0.048, n=4. (K) Immunostaining for C-peptide in d27 populations generated from the 38-2 iPS cell line. (L,M) Flow-cytometric analysis of C-peptide at d25 of differentiation in 38-2-derived untreated (Ctrl) and dorsomorphin-treated populations (Dorso). Percentages represent the mean percentage of cells expressing C-peptide (s.d.=1.3% for L, n=4; s.d.=0.6% for M, n=3).