Skip to main content
. Author manuscript; available in PMC: 2011 Sep 23.
Published in final edited form as: Neuron. 2010 Sep 23;67(6):953–966. doi: 10.1016/j.neuron.2010.08.044

Figure 5. SIRT1 Interacts with Tau.

Figure 5

(A) SIRT1 directly deacetylated ac-tau in vitro. Ac-tau/t-tau levels in the absence of immunoprecipitated SIRT1 were set as 1. Ac-tau was detected with Ab708 antibody. n=5 from two experiments. **, P=0.0063 (unpaired t test).

(B) GST pull-down assays. GST-tau protein (lanes 7–9) or GST alone (lanes 4–6) was incubated with lysates of cells transfected with FLAG-tagged SIRT1 or of nontransfected cells. Lanes 1, 4 and 7: 0.1% Triton X-100; lanes 2, 5, and 8: 0.5% Triton X-100; lanes 3, 6, and 9: 0.5% NP-40. Data shown are representative of two experiments. The lower band is likely to represent the cleavage product of SIRT1 observed previously (Ohsawa and Miura, 2006).

(C) Coimmunoprecipitation assays. HEK293T cells were transfected with a plasmid encoding FLAG-tagged human tau. Cell lysates were collected 24 h later, immunoprecipitated with an anti-FLAG antibody, and immunoblotted with Tau 5 or an anti-SIRT1 antibody. Lanes 1–2: input; lane 3: no primary antibody; lane 4: anti-FLAG antibody. Values are means ± SEM (A).