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. Author manuscript; available in PMC: 2012 Jan 17.
Published in final edited form as: Vaccine. 2010 Nov 21;29(4):721–727. doi: 10.1016/j.vaccine.2010.11.020

Figure 2. Production of cytokines by splenic cells.

Figure 2

Splenic cells were isolated from intranasally immunized mice at a time point 63 days after the initial immunization. Cultured splenic cells were stimulated in vitro for 4 days with AgI/II (5μg/ml). Culture supernatants from the stimulated splenic cells were measured for: (A) IL-2, (B) IL-6, (C) IFN-γ, (D) TNF-α, (E) IL-17, and (F) IL-10. Data are reported as arithmetic means; error bars denote one standard deviation of the mean (n = 5–6). Key: statistically different from mice immunized only with AgI/II at P<0.05 (*), P<0.01 (**), and P<0.001 (***).