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. Author manuscript; available in PMC: 2011 Feb 8.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2008 Feb;49(2):629–635. doi: 10.1167/iovs.07-1192

Figure 2.

Figure 2

Analysis of HTM 631, 682, and 701 cells grown on planar surfaces and on six-packs. The cells were stained with TRITC-labeled phalloidin, and then the number of cells that were elongated, oriented in the direction of the grooves and ridges, or perpendicular to the grooves and ridges were measured. Besides the flat surface, cells were grown on 400-, 800-, 1200-, 1600-, 2000-, and 4000-nm pitch surfaces. Elongation was generally very high, even on the planar surfaces. The cells were orienting to the ridges even at 400-nm pitch and the alignment increased to around 80% on the other pitched surfaces (±SEM).