Skip to main content
. Author manuscript; available in PMC: 2012 Jan 27.
Published in final edited form as: Neuron. 2011 Jan 27;69(2):244–257. doi: 10.1016/j.neuron.2010.12.016

Figure 3. CNS node of Ranvier organization requires NF186.

Figure 3

Cervical spinal cord sections from P3 (A-B, I-J), P6 (C-D; K-L), P11 (E-F; M-N), and P14 (GH; O-P) wild type (+/+) and Nefl-Cre;NfascFlox age-matched littermate mice were immunostained with antibodies against Caspr (green), AnkG (red, A′-H′) or Nav channels (panNav, red; I′-P′) where noted, plus NF186 (blue). Arrowheads mark the nodes lacking NF186 expression in the Nefl-Cre;NfascFlox mice. (Q-Q″) Quantification of the percentage of nodes lacking NF186 alone (-NF186, Q), lacking both NF186 and AnkG (Q′), and those lacking both NF186 and Nav channels (Q″) in P11 wild type (+/+, black bars) and Nefl-Cre;NfascFlox (gray bars) spinal cords. The percentage of nodes lacking both NF186 and AnkG [(−) AnkG] (Q′) or both NF186 and Nav channels [(−) pan-Nav] (Q″) are represented by the percent nodes lacking both proteins in relation to the total number of nodes lacking NF186. The graphs represent the average percentages calculated from three independent experiments, and the error bars represent the SEM. Asterisks (***) denote statistical significance with a p-value ≤ 0.0001. (See also Figs. S2, S3 and S6).