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. Author manuscript; available in PMC: 2011 Feb 8.
Published in final edited form as: Chem Biol. 2005 May;12(5):595–604. doi: 10.1016/j.chembiol.2005.04.007

Figure 7.

Figure 7

Lpg1 Competes with the Helicase for Binding to the Primase

Analytical gel filtration experiments of the DnaB (0.5 μM hexamers)-DnaG (0.67 μM monomers) mixtures in the absence (A) and presence (B) of lpg1 (175 μM). Samples from the peak fractions were analyzed by SDS-PAGE. The numbers of the lanes in the polyacrylamide gels correspond to the numbers of the peak fractions, as indicated. Lane M shows molecular weight markers, as indicated. lpg1 causes the dissociation of DnaG (67 kDa) from DnaB (50.5 kDa) in a competitive manner.