Collagen density distributions along the radial (a), transmural (b), and circumferential (c) directions in native PV with varied sectioning spacing (from 1 to 50 μm), and qualitative cell density distributions (based on cell nuclei measurements) along the radial (d), transmural (e), and circumferential (f) directions in the native PV. Inset image depicts distribution axes along central meridian of leaflet. Note the position-dependent variations in the radial and transmural directions while a relatively uniform distribution in the circumferential direction. In the collagen distributions, limited qualitative change exists can be observed with increased sectioning frequency, especially in the circumferential direction.