Table 1. Classification and general features of A. acidocaldarius 104-IA^T in accordance with the MIGS recommendations [33].

MIGS ID	Property	Term	Evidence code
	Current classification	Domain Bacteria	TAS [34]
		Phylum ‘Firmicutes’	TAS [35-37]
		Class Bacilli	TAS [36]
		Order Bacillales	TAS [38,39]
		Family ‘Alicyclobacillaceae’	TAS [40]
		Genus Alicyclobacillus	TAS [1]
		Species Alicyclobacillus acidocaldarius	TAS [21]
		Type strain 104-IA	TAS [21]
	Gram stain	positive	TAS [21]
	Cell shape	small rods	TAS [1]
	Motility	not reported (relevant genes missing)	NAS
	Sporulation	refractile endospores	TAS [21]
	Temperature range	45°C-70°C	TAS [21]
	Optimum temperature	60°C-65°C	TAS [21]
	Salinity	does not grow with 5% (w/v) NaCl	TAS [22]
MIGS-22	Oxygen requirement	strictly aerobic	TAS [21]
	Carbon source	saccharolytic	TAS [21]
	Energy source	carbohydrates	TAS [21]
MIGS-6	Habitat	hot acidic springs and soil	TAS [21]
MIGS-15	Biotic relationship	free living	NAS
MIGS-14	Pathogenicity	none	NAS
	Biosafety level	1	TAS [41]
	Isolation	acid hot spring	TAS [21]
MIGS-4	Geographic location	Nymph Creek, Yellowstone National Park, USA	TAS [21]
MIGS-5	Sample collection time	about 1970	TAS [21]
MIGS-4.1	Latitude	44.376	NAS
MIGS-4.2	Longitude	110.690	NAS
MIGS-4.3	Depth	not reported
MIGS-4.4	Altitude	not reported

Evidence codes - IDA: Inferred from Direct Assay (first time in publication); TAS: Traceable Author Statement (i.e., a direct report exists in the literature); NAS: Non-traceable Author Statement (i.e., not directly observed for the living, isolated sample, but based on a generally accepted property for the species, or anecdotal evidence). These evidence codes are from of the Gene Ontology project [42]. If the evidence code is IDA, then the property was directly observed for a living isolate by one of the authors or an expert mentioned in the acknowledgements.