Fig. 2.
Multiplex three-primer PCR strategy for simultaneous detection of wild-type and deleted mtDNA. Wild-type mtDNA is amplified using the forward and reverse primers F1 and R1, respectively; amplicon detection accomplished with the molecular beacon MB1. Deleted mtDNA (indicated by the dashed line) is amplified using the forward and reverse primers F1 and R2, respectively; amplicon detection is accomplished with the molecular beacon MB2. OH and OL indicate the origin of replication of the heavy and light DNA strands, respectively. Sequences for F1, F2, R1, MB1, and MB2 are shown in Table 1. Other details are published elsewhere [14]