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| Position | Sequence | |
|---|---|---|
| Primers | ||
| Forward (F1) | 7,798–7,820 | 5′-CATCCTAGTCCTCATCGCCCTCC |
| Reverse 1 (R1) | 8,186–8,209 | 5′-GGGCATGAAACTGTGGTTTGCTCC |
| Reverse 2 (R2) | 15,727–15,749 | 5′-GAATGAGGAGGTCTGCGGCTAGG |
| Molecular beacons | ||
| MB1a | 8,072–8,092 | FAM-5′-CCAGCG GCCTAATGTGGGGA CAGCTCACGCTGG-BHQ1 |
| MB2a | 15,534–15,553 | HEX-5′-CCAGCG CTTGATGTGGGGAG GGGTGTCGCTGG-BHQ1 |
See Fig. 2 for primer binding details.
The stem sequence is shown in bold.
