Table 2. Real-time PCR amplification of wild-type and deleted mtDNA from mitochondria collected after laser-induced fluorescence detection.

No. of mtDNA moleculesa	Total PicoGreen signal	Deleted mtDNA (%)b	No. of collected mitochondria
0	0.00	100.0	0
0	0.00	100.0	0
0	0.00	0.0	0
0	0.00	100.0	0
3	0.06	0.0	1
3	0.03	100.0	1
3	0.06	0.0	1
4	0.07	0.0	2
6	0.06	100.0	2
7	0.12	0.0	1
8	0.08	100.0	2
8	0.14	0.0	3
10	0.18	0.0	2
10	0.18	0.0	2
10	0.18	0.0	3
11	0.11	100.0	1
18	0.17	100.0	2
18	0.18	100.0	3
20	0.35	0.0	1
23	0.23	100.0	5
24	0.23	100.0	6
25	0.31	66.7	6
27	0.26	100.0	8
28	0.27	100.0	2
28	0.46	18.2	6
35	0.34	100.0	4
38	0.37	100.0	7
39	0.69	0.0	2
40	0.59	39.7	9
42	0.27	100.0	5
46	0.45	100.0	3
50	0.49	100.0	5
57	0.64	84.1	7
58	1.05	0.0	7
59	0.58	100.0	7
73	0.72	100.0	6
79	1.04	59.3	7
83	0.97	76.4	6
88	0.87	100.0	4
90	0.91	97.1	8
100	1.80	0.0	5
101	1.00	100.0	4
107	1.29	73.3	8
122	1.20	100.0	13
126	1.25	98.9	12
146	2.62	0.0	9
148	2.66	0.0	11
154	1.52	100.0	3
157	1.70	87.8	7
170	1.92	82.1	9
175	1.73	100.0	12
181	3.26	0.0	1
192	2.24	78.0	11
208	2.18	92.1	12
209	3.76	0.0	2
210	2.07	100.0	9
239	2.57	88.6	16
295	3.45	77.4	8
415	4.46	88.8	21
420	4.37	93.0	5
430	4.27	98.9	11
537	6.44	73.6	12
615	6.06	100.0	10
617	6.61	89.4	7
664	7.47	82.8	7
808	8.99	84.3	15
811	8.25	95.8	29
822	8.56	92.9	32
988	17.78	0.0	8
1,516	16.62	86.3	46
1,818	20.35	83.4	64
2,001	22.16	84.9	64

Mitochondria were isolated from ΔH2-1 cybrid cells, harboring a heteroplasmic mtDNA deletion, and labeled with PicoGreen. Mitochondria were hydrodynamically mobilized through a capillary and detected on-column using laser-induced fluorescence. Mitochondria were then collected from the outlet of the capillary, and real-time PCR was performed to amplify the wild-type and deleted mtDNA. Of the collected samples, 35% were PCR negative

^aThe number of total mtDNA molecules was determined using Eq. 2

^bItalics indicate samples displaying heteroplasmy