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. 2011 Jan 26;31(4):1323–1332. doi: 10.1523/JNEUROSCI.5383-10.2011

Figure 5.

Figure 5.

Knockdown of endogenous rat AKAP150 increases Kv4.2 surface expression in hippocampal neurons. A, Cultured rat embryonic day 18 (E18) hippocampal neurons were transfected with control siRNA (siCtrl) or siAKAP150 by using the Nucleofector System. Biotinylation assay shows that surface level of endogenous Kv4.2 is significantly increased by knock-down of endogenous AKAP150 by siAKAP150 compared with siCtrl. GAPDH served as a loading control. β-Actin is labeled as negative surface control. Surface Kv4.2 level is increased ∼30% by siAKAP150 knock-down (n = 5, p < 0.05). B, As with the biotinylation results, electrophysiological recordings from cultured hippocampal neurons showed peak current density of Kv4.2-mediated currents also significantly increased (∼1.5-fold) by knock-down endogenous AKAP150 with siAKAP150 (n = 12) compared with siCtrl (n = 15). Calibration: 200 pA, 200 ms.