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. Author manuscript; available in PMC: 2011 Feb 8.
Published in final edited form as: Chem Commun (Camb). 2010 May 13;46(30):5542–5544. doi: 10.1039/c0cc00423e

Fig. 2.

Fig. 2

Secondary structures for the 27-base RNA models of the 16S and 18S A-sites. U1406 of the 16S A-site is replaced with an isosteric emissive nucleoside analogue as a donor (F1); the place-holding molecule is tagged with an appropriate fluorophore (F2); the 18S A-site is tagged with an acceptor (F3) to match the labeled “place-holder” (F2). The affinity and selectivity of unlabeled small-molecules for either A-sites can be accurately monitored using FRET, as the place-holder is displaced.