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. Author manuscript; available in PMC: 2011 Feb 8.

Published in final edited form as: Chem Commun (Camb). 2010 May 13;46(30):5542–5544. doi: 10.1039/c0cc00423e

Fig. 5 — Fractional fluorescence saturation of the donor F1 (■) in the labeled 16S A-site, the emissive fluorophore F2 () tagged to kanamycin A, and the emissive acceptor F3 (○) of the 18S A-site in studying the binding of: (top) negamycin; (bottom) neamine. Conditions: 16S RNA (5 × 10⁻⁷ M), 18S RNA (5 × 10⁻⁷ M), coumarin-labeled-kanamycin A (2.2 × 10⁻⁶ M), cacodylate buffer pH 7.0 (2.0 × 10⁻² M), NaCl (1.0 × 10⁻¹ M).¹³

Inline graphic — Fractional fluorescence saturation of the donor F1 (■) in the labeled 16S A-site, the emissive fluorophore F2 () tagged to kanamycin A, and the emissive acceptor F3 (○) of the 18S A-site in studying the binding of: (top) negamycin; (bottom) neamine. Conditions: 16S RNA (5 × 10⁻⁷ M), 18S RNA (5 × 10⁻⁷ M), coumarin-labeled-kanamycin A (2.2 × 10⁻⁶ M), cacodylate buffer pH 7.0 (2.0 × 10⁻² M), NaCl (1.0 × 10⁻¹ M).¹³