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. 2010 Nov 9;39(3):e12. doi: 10.1093/nar/gkq1070

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — TK as an OFF-selector. (A) Mechanism of action. dP travels through the cell membrane, is phosphorylated by hsvTK, and is incorporated into genomic DNA. P can pair with both A and G, elevating the mutation rate within the cell. Having their genetic information damaged rapidly, cells quickly die of lethal mutagenesis. (B) The number of viable cells after 12 h incubation in the presence of dP (0–100 nM). Filled circles represent BL21 (tdk) expressing hsvTK; open circles represent BL21 (tdk) without hsvTK expression.