Figure 6.

Competition of two inverter circuits. The mixture of Strain-A and -B was subjected to OFF selection in the presence of 100 nM AHL, followed by ON selection in its absence. The existing ratio of the Plasmids-A and -B was tracked by PCR of the culture after overnight growth in selection media. (A) Annealing sites of the primers used for PCR analysis to the Plasmid-A and -B. (B) Gel electrophoresis of the PCR products taken from each step of the selection procedure. Lane: 1, culture containing Strain-A; 2, culture containing Strain-B; 3, mixed culture of Strain-A and -B before selection; 4, mixed culture after OFF selection; and 5, mixed culture after the subsequent ON selection.