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. 2010 Sep 28;39(3):1066–1080. doi: 10.1093/nar/gkq748

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Comparison of A-minor junctions from bacteria, archaea and eukaryotes. Variants of the H68-H70 A-minor junctions based on the natural sequences from H. marismortui (H.m), S. cerevisiae (S.c), E. coli (E.c) and T. Thermophilus (T.t) 23S rRNAs are compared by PAGE analysis. The receptors were designed by the method illustrated in Figure 5A. Additionally, two mutant H68–H70 junctions were prepared to allow all the receptors to be bridged by a series of single-point mutations. PAGE gels were run under the same conditions as those shown in the previous figures.