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. 2010 Sep 24;39(3):1054–1065. doi: 10.1093/nar/gkq850

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Morphological and genetic changes of mirPS cell properties in response to different mir-302 concentrations. (A) Morphological changes of mirPS cell colonies in response to Dox-induced mir-302 expression. Mir-434-expressing cells generated by electroporating hHFCs with pTet-On-tTS-miR434-5p were served as a negative control. A morphological comparison between a morula-staged rat embryo and a mirPS cell colony at 32–64 cell stage was shown in the lower panel. (B) Fluorescent microscope examination showing strong and homogeneous expression of the core pluripotent stem cell markers Oct3/4, Sox2 and Nanog in a mirPS-derived embryoid body. BF-DIC, bright field with differential interference contrast. (C) Western blotting confirming the expression patterns of major hES-specific marker proteins in completely (10 µM Dox) and partially (5 µM Dox) reprogrammed mirPS cells compared to those found in hES H1 and H9 cells (n = 4, P < 0.01). Mir-302 expression was proportional to Dox concentration.