Figure 6.

EXO1 is not required for tdp1Δmediated stimulation of joining blunt ends. (A) YCplac111 digested with SmaI was transfected into WT, exo1Δ, tdp1Δ or exo1Δ tdp1Δ strains. Repair frequencies were normalized with transfection of undigested YCplac111. The results shown are the mean of at least three independent transfections; error bars indicate SEM. (B) Plasmids were recovered from 100 independent colonies from wild-type, exo1Δ, tdp1Δ and exo1Δ tdp1Δ mutant strains. Data from the wild-type and single mutants were as presented in Figure 4B. As in Figure 4B, each dot represents a single isolate with the indicated deletion size, and accurately repaired junctions are not presented.