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The effect of protein synthesis inhibitor, cycloheximide (CHX) on IL-1β-induced increase in MLCK protein expression and drop in Caco-2 TER. A, Cycloheximide (5 μM) pretreatment prevented the IL-1β-induced up-regulation in MLCK protein expression as assessed by Western blot analysis. B, Cycloheximide prevented the IL-1β-induced drop in Caco-2 TER (means ± SE, n = 4). *, p < 0.001 vs control; **, p < 0.001 vs IL-1β treatment.
