Skip to main content
. Author manuscript; available in PMC: 2011 Feb 8.
Published in final edited form as: J Immunol. 2008 Apr 15;180(8):5653–5661. doi: 10.4049/jimmunol.180.8.5653

FIGURE 3.

FIGURE 3

Effect of IL-1β on Caco-2 MLCK activity. A, Time course effect of IL-1β on MLCK activity of filter-grown Caco-2 monolayers was determined by ELISA-based in vitro kinase activity measurements as described in Materials and Methods. After appropriate time period, MLCK activity was assessed by measuring the in vitro phosphorylation of MLC (S-19). IL-1β treatment resulted in a time-dependent increase in Caco-2 MLCK activity. *, p < 0.05 vs control. B, Time course effect of IL-1β on phosphorylation of MLC (P-MLC) in filter-grown Caco-2 monolayers over the 48-h experimental period as determined by Western blot analysis. C, Pretreatment with cycloheximide (5 μM) inhibited the IL-1β-induced increase in MLCK kinase activity, cycloheximide alone did not affect MLCK enzyme activity. *, p < 0.001 vs control; **, p < 0.001 vs IL-1β treatment.