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Effect of siRNA induced MLCK knock-down on IL-1β-induced drop on Caco-2 TER. Caco-2 monolayers were transfected with MLCK siRNA for a 96-h time period as described in Materials and Methods. A, MLCK siRNA transfection resulted in a near complete depletion in MLCK protein expression as determined by Western blot analysis. B, MLCK siRNA transfection prevented the IL-1β-induced drop in Caco-2 TER (n = 4). *, p < 0.001 vs control; **, p < 0.001 vs IL-1β treatment.
