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. Author manuscript; available in PMC: 2011 Feb 8.
Published in final edited form as: J Immunol. 2008 Apr 15;180(8):5653–5661. doi: 10.4049/jimmunol.180.8.5653

FIGURE 6.

FIGURE 6

IL-1β effect on Caco-2 MLCK mRNA expression. A, Time course effect of IL-1β on Caco-2 MLCK mRNA expression was determined by real-time PCR as described in the Materials and Methods. MLCK mRNA level was expressed relative to the control level which was assigned a value of 1. The average copy number of MLCK mRNA in controls was 4.63 × 1011. *, p < 0.001 vs control. B, Actinomycin-D (100 ng/ml) effect on IL-1β-induced increase in MLCK mRNA expression (6 h of IL-1β treatment). Actinomycin-D significantly inhibited the IL-1β increase in MLCK mRNA expression. *, p < 0.05 vs control; **, p < 0.05 vs IL-1β treatment. C, Actinomycin-D significantly prevented the IL-1β-induced up-regulation of MLCK protein expression as determined by Western blot analysis. D, Actinomycin-D also prevented the IL-1β-induced drop in Caco-2 TER (n = 4). *, p < 0.001 vs control; **, p < 0.001 vs IL-1β treatment.