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. Author manuscript; available in PMC: 2011 Feb 8.
Published in final edited form as: J Immunol. 2008 Apr 15;180(8):5653–5661. doi: 10.4049/jimmunol.180.8.5653

FIGURE 8.

FIGURE 8

Effect of siRNA induced knock-down of NF-κB p65 on IL-1β-induced increase in MLCK expression and drop in Caco-2 TER and. Filter-grown Caco-2 monolayers were transfected with NF-κB p65 siRNA for a 96-h time period as described in Materials and Methods. A, NF-κB p65 siRNA transfection resulted in a near complete knock-down of NF-κB p65 expression. B, siRNA NF-κB p65 transfection prevented the IL-1β-induced increase in MLCK protein expression. C, NF-κB p65 siRNA transfection prevented the IL-1β-induced drop in Caco-2 TER. *, p < 0.001 vs control; **, p < 0.001 vs IL-1β treatment.