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Example ITC data from an experiment measuring the association of two single-stranded 7-mer RNAs. Data collected at 15 °C in 50mM HEPES, pH 7.5, and 1 M NaCl. Top panel. Power versus time curve. Bottom panel. Integrated injection enthalpy plotted versus the mole ratio of the reactants. ΔH = −46.1 ± 0.2 kcal/mol, Ka = 4.3 × 10−7 M and n = 0.91 ± 0.1 (Reprinted with permission from Takach et al. (2004). Copyright, American Chemical Society.)
