Fig. 2.

A: Effect of fasting and postprandial plasma (from volunteer 7135) on the EPR spectra of spin- labeled apoE3-like protein and apoE4. ApoE3-like or apoE4 were incubated with pre- or postprandial plasma at 37°C for 1 h before scanning. Each spectrum represents the same number of spins from a spin-labeled apoE3 or apoE4 at a concentration of 0.2 mg/ml. B: Distribution of spin-labeled apoE3-like or apoE4 associated with plasma lipoproteins. The protein samples were incubated at 37°C for 1 h with fasting plasma samples from healthy human subjects. The lipoproteins were separated by gel electrophoresis and stained with Fat Red 7b. The VLDL, LDL, and HDL bands were excised and solubilized into 4.5 mol/l guanidine isocyanate by incubating at 65°C for 3 min. The gel extracts were subjected to EPR spectroscopy. Data represent the average of six independent measurements. Error bars represent SD. *P < 0.001 for significantly different treatments within each apoE (E3/E4) protein group. ^#P < 0.001 for significantly different treatments within each lipoprotein class.