Fig. 5.

High-fat n-3 PUFA diet does not modify the percentage of activated B cells but increases CD69 surface expression and secretion of IL-6 and IFNγ. (A) Percentage of B cells activated after 24 h of activation and (B) MFI of CD69, MHC class II, and CD80 of B cells isolated from mice fed ND, SFA, HFA, MUFA, and n-3 PUFA diets. (C) Cytokine secretion from B220⁺ B cells isolated from diet modified mice. Data are n = 3 (A) and n = 4 (B–C) per diet. Asterisks indicate different from ND, *P < 0.05, **P < 0.01. HFA, hydrogenated fatty acid; MUFA; monunsaturated fatty acid ND, normal diet; PUFA; polyunsaturated fatty acid SFA, saturated fatty acid.