High-fat n-3 PUFA diet increases body weight gain and induces splenomegaly. (A) Body weight as a function of time for mice fed ND or high-fat diets enriched in SFA, HFA, MUFA, and n-3 PUFA. Average daily (B) food and (C) kcal intake of mice fed the different diets as a function of time. The kcal values are normalized to the body weight. (D) Spleen weight of mice fed the different diets after 98 days of feeding. Values are mean ± SEM, n = 6 per diet. Asterisks indicate difference from ND, *P < 0.05, **P < 0.01, ***P < 0.001. HFA, hydrogenated fatty acid; MUFA; monunsaturated fatty acid ND, normal diet; PUFA; polyunsaturated fatty acid SFA, saturated fatty acid.