TABLE 2.
Treatment of naïve B cells with n-3 PUFA for 48 h improves the n-6/n-3 PUFA ratio
| Fatty Acid | BSA | PA | OA | ELA | EPA | DHA | PA+OA | PA+ELA | PA+EPA | PA+DHA |
|---|---|---|---|---|---|---|---|---|---|---|
| 14:0 | 1.9 ± 0.3 | 0.9 ± 0.2 | 1.4 ± 0.1 | 1.3 ± 0.1 | 1.6 ± 0.4 | 2.2 ± 0.4 | 0.8 ± 0.2 | 0.8 ± 0.1 | 1.0 ± 0.1 | 0.9 ± 0.1 |
| 16:0 | 32.1 ± 0.6 | 37.8 ± 0.5a | 23.9 ± 0.4b | 16.0 ± 1.4c | 29.2 ± 3.9 | 39.2 ± 2.5a | 33.4 ± 0.5 | 28.7 ± 0.7 | 42.2 ± 0.5c | 41.4 ± 0.2c |
| 16:1 | 13.0 ± 0.4 | 11.3 ± 0.6 | 1.4 ± 0.2c | 5.8 ± 0.7c | 1.1 ± 0.4c | 2.4 ± 0.3c | 6.7 ± 0.5c | 13.5 ± 0.8 | 3.3 ± 0.2c | 4.2 ± 0.2c |
| 18:0 | 11.5 ± 0.6 | 10.2 ± 0.6 | 7.7 ± 0.4 | 4.7 ± 0.3 | 12.4 ± 2.5 | 9.9 ± 0.5 | 7.9 ± 0.4 | 5.5 ± 0.5 | 8.3 ± 0.4 | 9.0 ± 0.8 |
| 18:1t | 0.2 ± 0.1 | 0.2 ± 0.0 | 0.3 ± 0.1 | 43.4 ± 3.2c | 0.5 ± 0.2 | 0.1 ± 0.0 | 0.3 ± 0.1 | 23.6 ± 0.8c | 0.4 ± 0.2 | 0.0 ± 0.0 |
| 18:1c | 17.8 ± 0.2 | 12.4 ± 1.0 | 45.9 ± 0.8c | 11.4 ± 0.8 | 11.1 ± 3.1 | 5.6 ± 0.3 | 33.9 ± 0.5c | 11.0 ± 0.4 | 3.7 ± 0.3 | 4.7 ± 0.2 |
| 18:2 n-6 | 4.9 ± 0.3 | 8.4 ± 2.5 | 3.0 ± 04 | 4.0 ± 0.3 | 10.4 ± 5.3 | 2.7 ± 0.1 | 2.8 ± 0.2 | 4.5 ± 0.5 | 1.9 ± 0.2 | 2.0 ± 0.1 |
| 18:3 n-6 | 0.5 ± 0.1 | 0.5 ± 0.2 | 2.6 ± 0.1 | 0.2 ± 0.2 | 0.1 ± 0.0 | 0.0 ± 0.0 | 1.2 ± 0.0 | 0.2 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 |
| 18:3 n-3 | 0.1 ± 0.3 | 0.2 ± 0.0 | 0.1 ± 0.0 | 0.2 ± 0.0 | 0.6 ± 0.4 | 0.1 ± 0.1 | 0.1 ± 0.0 | 0.2 ± 0.1 | 0.1 ± 0.0 | 0.1 ± 0.0 |
| 20:0 | 0.2 ± 0.0 | 0.4 ± 0.0 | 0.1 ± 0.0 | 0.2 ± 0.0 | 0.3 ± 0.2 | 0.1 ± 0.0 | 0.2 ± 0.0 | 0.0 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 |
| 20:1 | 0.4 ± 0.1 | 0.2 ± 0.0 | 0.3 ± 0.0 | 0.2 ± 0.1 | 0.3 ± 0.1 | 0.1 ± 0.0 | 0.2 ± 0.1 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.1 ± 0.0 |
| 20:2 n-6 | 0.4 ± 0.1 | 0.2 ± 0.0 | 0.8 ± 0.0 | 0.7 ± 0.1 | 0.4 ± 0.1 | 0.3 ± 0.1 | 0.5 ± 0.1 | 0.4 ± 0.0 | 0.3 ± 0.0 | 0.1 ± 0.0 |
| 22:0 | 0.6 ± 0.1 | 0.9 ± 0.1 | 0.4 ± 0.0 | 0.3 ± 0.0 | 0.8 ± 0.2 | 0.3 ± 0.1 | 0.4 ± 0.0 | 0.4 ± 0.1 | 0.4 ± 0.1 | 0.4 ± 0.1 |
| 22:1 | 0.2 ± 0.0 | 0.1 ± 0.1 | 0.4 ± 0.0 | 0.0 ± 0.0 | 0.4 ± 0.1 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.2 ± 0.1 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| 20:4 n-6 | 8.6 ± 0.7 | 10.0 ± 1.3 | 5.8 ± 0.4a | 6.6 ± 0.4 | 3.6 ± 0.7c | 5.0 ± 0.2b | 6.3 ± 0.1 | 6.4 ± 0.3 | 3.4 ± 0.2c | 4.1 ± 0.1b |
| 20:5 n-3 | 0.6 ± 0.1 | 0.6 ± 0.1 | 0.4 ± 0.0 | 0.4 ± 0.1 | 15.0 ± 1.4c | 1.9 ± 0.2 | 0.4 ± 0.1 | 0.4 ± 0.1 | 18.1 ± 0.5c | 0.8 ± 0.1 |
| 24:0 | 0.5 ± 0.1 | 0.6 ± 0.0 | 0.2 ± 0.0 | 0.3 ± 0.0 | 0.4 ± 0.1 | 0.3 ± 0.0 | 0.3 ± 0.0 | 0.4 ± 0.1 | 0.2 ± 0.1 | 0.4 ± 0.0 |
| 24:1 | 0.7 ± 0.1 | 0.3 ± 0.1 | 1.5 ± 0.1 | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.3 ± 0.0 | 0.9 ± 0.1 | 0.2 ± 0.0 | 0.3 ± 0.1 | 0.4 ± 0.1 |
| 22:5 n-3 | 2.0 ± 0.1 | 1.8 ± 0.2 | 1.4 ± 0.1 | 1.4 ± 0.1 | 10.4 ± 6.4 | 0.9 ± 0.0 | 1.2 ± 0.1 | 1.2 ± 0.0 | 15.5 ± 0.8c | 0.7 ± 0.1 |
| 22:6 n-3 | 3.8 ± 0.4 | 3.0 ± 0.5 | 2.4 ± 0.2 | 2.6 ± 0.1 | 1.1 ± 0.2 | 28.5 ± 0.5c | 2.4 ± 0.0 | 2.4 ± 0.1 | 0.7 ± 0.1 | 30.5 ± 0.1c |
| ΣSFA | 46.8 ± 1.5 | 50.8 ± 2.3 | 33.7 ± 0.7c | 22.8 ± 1.7c | 44.7 ± 1.5 | 52.0 ± 1.0 | 43.0 ± 0.6 | 35.8 ± 0.8c | 52.2 ± 1.1 | 52.2 ± 1.6 |
| ΣMUFA | 32.3 ± 0.6 | 24.5 ± 1.5c | 49.8 ± 0.6c | 61.1 ± 1.9c | 13.7 ± 2.5c | 8.6 ± 0.3c | 42.1 ± 0.6c | 48.5 ± 1.0c | 7.7 ± 0.3c | 9.4 ± 0.3c |
| ΣPUFA | 20.9 ± 1.5 | 24.7 ± 1.5 | 16.5 ± 0.3 | 16.1 ± 1.0 | 41.6 ± 2.2c | 39.4 ± 3.0c | 14.9 ± 0.3a | 15.7 ± 0.2 | 40.1 ± 0.8c | 38.4 ± 1.0c |
| n-6/n-3 | 2.2 ± 0.1 | 3.4 ± 0.5b | 2.8 ± 0.2 | 2.5 ± 0.1 | 0.5 ± 0.5c | 0.3 ± 0.1c | 2.6 ± 0.1 | 2.7 ± 0.1 | 0.2 ± 0.0c | 0.2 ± 0.0c |
B cells were isolated from the spleens of C57BL/6 mice and activated with LPS and treated with fatty acids (50 µM each). Extracted lipids were then analyzed via GC. Values are average ± SEM, n = 4. Asterisks indicate difference from BSA.
Abbreviations: DHA, docosahexaenoic acid; ELA, elaidic acid; EPA, eicosapentaenoic acid; GC, gas chromatography; LPS, lipopolysaccharide; OA, oleic acid; PA, palmitic acid.
a
P < 0.05.
b
P <0.01.
c
P <0.001.