Fig. 10.

DHL, but not lanosterol, is an LXR ligand. A: Coactivator recruitment assay of LXRα and LXRβ by FRET analysis. Dose-response curves of LXRα and LXRβ activation by DHL and lanosterol compared with EPCH. Data are means + SEM of two independent assays. B: The 10 T1/2 cells were transiently transfected with a GAL4-responsive luciferase reporter and an expression plasmid encoding a chimeric fusion of the GAL4 DNA binding domain and the LBD of hLXRα. Transfected cells were treated for 6 h with either the synthetic LXR ligand GW3965 (1 µM) or the sterols EPCH (9 µM), lanosterol (9 µM), or DHL (9 µM). Normalized luciferase values are expressed as fold induction versus DMSO; error bars indicate SDs. **P values < 0.01 compared with vehicle. All compounds tested except lanosterol activate GAL4-LXRα.