Fig. 3.

Accumulation and characterization of an HA-FoxO4-specific lipid. A: TLC plate stained with iodine vapor showing fractionation of lipid extracts from Puro and HA-FoxO4 cells. Each lane is from a single well of a 6-well plate. HA-FoxO4 extracts show two additional unknown lipids, UL1 and UL2. B: TLC plate autoradiograph of lipid extracts from Puro and HA-FoxO4 cells showing de novo synthesis of ¹⁴C-labeled cholesterol (chol) in Puro cells and the ¹⁴C-unknown lipid (UL1) in HA-FoxO4 cells. C: Densitometric scan of cholesterol and the HA-FoxO4 lipid from the autoradiograph shown in B. **P < 0.01. Lipid markers are Cholesterol (chol), oleic acid (OA), and cholesteryl oleate, a cholesteryl ester (CE).