Fig. 5.

Accumulation of lipid droplets and determination of their protein coating in Puro and HA-FoxO4 cells. A: Nile red fluorescence staining of lipid droplets in HA-FoxO4 and Puro fibroblasts (200×). B: Western blots of protein lysates from fibroblasts (Puro and HA-FoxO4), white adipose tissue (WAT), 3T3L1 preadipocytes, and a mixture or 3T3L1 preadipocytes and adipocytes following induced differentiation. The Western blots were conjugated to antiperilipin, anti-adipocyte related protein (ADRP), or anti-tubulin (for protein loading).