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Uptake of 3H-2 deoxy glucose (3H-2DG) in Puro (P) and HA-FoxO4 (F) fibroblasts. Basal uptake (A) or pretreatments( B) with 50–100 nM insulin, 10 μM cytochalasin B (C), 50 μM LY294002 (LY) (D), 50 μM PD98059 rapamycin, or 1 μM rapamycin (Rapa) (E) prior to 3H-2DG uptake (n = 6 wells per group). Values are normalized to the protein content of each well. **P value < 0.01.
