TABLE 1.

DNA sequence of PCR primers used for real-time amplification of selected genes in cholesterol biosynthesis

Gene	Forward Primer	Reverse Primer
HMG-CoA synthase	5′-TGGAGACCACAGTTCTCTGTCCTT-3′	5′-GCAACGATTCCCACATCTTTTGGC-3′
HMG-CoA reductase	5′-GCGAGTGCATTAGCAAAGTTTGCC-3′	5′-GCATTCCACAAGAGCGTCAAGAGT-3′
Isopentenyl diphosphate isomerase	5′-CGCTTGAAAGCCGAGTTGGGAATA-3′	5′-TGTTCACCCCAGATACCATCAGA-3′
SREBP2	5′-AGCTGTGCGCTCTCGTTTTACT-3′	5′-AGGCATGCATGGCTCTACAGGTAT-3′
Δ24 sterol reductase	5′-GCTGCGAGTCGGAAAGTACAAGAA-3′	5′-TGTCACCTGACCCATAGACACCAA-3′
Lanosterol demethylase (CYP51)	5′-ACGCAGACCTGGATGGAGGTTTTA-3′	5′-TCTTTTGACAGCCTGCGCTTCT-3′