Fig. 4.

Hepatic de novo lipogenesis is regulated by diet composition at the transcriptional level. Transcript levels of fatty acid synthase (fasn; A), acetyl-CoA carboxylase 1 (acc1; B), sterol regulatory element-binding proteins 1 (srebp1; C), and carbohydrate response element binding protein (chrebp; D) were quantified by real-time PCR in the liver of Wistar rats fed either low-fat/high-carbohydrate diet (HCD; open bars), high-fat diet (HFD; hatched bars), or Western diet (WD; black bars) for acute term (AT), short term (ST), intermediate term (IT), or long term (LT). Baseline mRNA levels (B; gray bar) were determined from 8-week-old rats fed standard rodent chow for 2 weeks before the beginning of the feeding protocol. Data are means ± SE of n = 13–17 animals per group per time point (except baseline; n = 7). One, two, and three symbols represent P < 0.05, P < 0.01, and P < 0.001, respectively. # = vs. WD at same age; $ = vs. HFD at same age; a = vs. baseline; b = vs. acute term; c = vs. short term; d = vs. intermediate term.